Expression of bacterial adhesins in E.coli: From mapping of adhesive epitopes to structure
نویسندگان
چکیده
Cover figure: A stereo view of the surface of the GafD1-178 showing the bound GlcNAc (courtesy of Michael Merckel) CONTENTS LIST OF ORIGINAL PUBLICATIONS SUMMARY 1. INTRODUCTION 1 1.1. The diversity of bacterial adhesins 1 1.2. Bacterial adhesins mediate attachment to a variety of targets 2
منابع مشابه
Antigenic and immunogenic evaluation of Helicobacter pylori FlaA epitopes
Objective(s): Helicobacter pyloriare among most common human pathogens affecting at least half of the world’s population. Mobility is one of the important primary factors in bacterial colonization and invasion. The purpose of this research is cloning, expression, and purification of FlaA protein specific epitopes in order to evaluate their antigenicity and immunogenicity. Materials and Methods:...
متن کاملThe chaperone-usher pathway of bacterial adhesin biogenesis -- from molecular mechanism to strategies of anti-bacterial prevention and modern vaccine design.
The chaperone-usher system determines the biogenesis of surface-exposed adhesive structures responsible for virulence of many Gram-negative bacteria. Investigations of the last 20 years have resolved the mechanism of this pathway on a structural level for different species of pathogenic bacteria. The purpose of this review is to present the molecular mechanisms of the biogenesis of adhesive str...
متن کاملImmunoinformatics Design of a Multi-epitope-based Vaccine Against Colorectal Cancer
Background: Bioinformatic approaches for designing vaccines have become a promising alternative to conventional methods. We herein designed a multi-epitope-based vaccine against colorectal cancer (CRC). Methods: Used peptides in the CRC vaccines were retrieved from databases of PubMed, Web of Science, Google Scholar, and Clinical trials. The adjuvants of Mycobacterial heparin-binding hemagglut...
متن کاملDesigning highly effective and genetically invert fragments by full assessment of mutations in seed region siRNAs in omega gliadin epitopes
RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...
متن کاملCloning and Codon-optimized Expression of Structural Protein Hypervariable Region of VP2 from Infectious Bursal Disease Virus
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. Structural protein VP2 of IBDV is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The objective of the present study was to improve the expression of hypervariable re...
متن کامل